San Antonio Missions National Historical Park Boundary Expansion Act of 2010

San Antonio Missions National Historical Park Expansion Act of 2010 - Directs the Secretary of the Interior to study lands within Bexar and Wilson Counties in Texas, to identify lands that would be suitable for inclusion in the San Antonio Missions National Historical Park.

Authorizes the Secretary: (1) to enter into a cooperative agreement with the city of San Antonio or its designee for the operation of a facility outside the boundary of the Park that would provide visitor facilities and office space for a headquarters and operational support for the Park; and (2) to install in such facilities the cooperative agreement exhibits related to the resources at the Park.

Modifies the boundary of the Park to include approximately 151 acres of additional land, as identified on the map entitled "San Antonio Missions National Historical Park Proposed Boundary Addition 2009," dated November 2009. Bars the use of condemnation authority by the Secretary for the acquisition of lands and interests under this Act.